Coding

Part:BBa_K5157013:Design

Designed by: Yueheng Niu   Group: iGEM24_Jiangnan-China   (2024-09-26)


Tr linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A short sequence originated from Trichoderma reesei [1], being used as a flexible linker to link two structural domains of a fusion protein to achieve proper separation of functional domains to reduce their unfavorable interactions [2].

Source

A short sequence originated from Trichoderma reesei [1].

Reference

[1] Dai L, Qu Y, Huang J W, et al. Enhancing PET hydrolytic enzyme activity by fusion of the cellulose-binding domain of cellobiohydrolase I from Trichoderma reesei [J]. Journal of Biotechnology, 2021, 334: 47-50.
[2] Chen X, Zaro J L, Shen W C. Fusion protein linkers: property, design and functionality [J]. Advanced Drug Delivery Reviews, 2013, 65(10): 1357-1369.